Custom DNA constructs
Project cost and payment
Q: How is my project cost determined?
A: Guidelines for standard cloning costs are provided here. Cloning to viral vectors can increase the cost by $100. Presence or repetitive or high GC sequences, cloning involving large sequences or multiple changes may also increase the cost.
Sending source material and receiving constructed plasmids
Q: How and where should I send my source plasmids?
A: Click here
Q: What data will you need to determine the cost and to execute the project?
A: Full sequence of your destination plasmid and description of the changes you want made (mutagenesis; deletions, tags addition, insert cloning etc.). For insert cloning please send the sequence of the insert, or, better, the complete sequence of the source plasmid for the insert. You can also describe the required changes by simply sending the full sequence of the desired final plasmid. If you don't have complete sequences send the information you have (maps, partial sequences etc.).
Q: What form of plasmid and documentation will I receive?
A: Plasmids are prepared as miniprep DNA, a small amount, sufficient for multiple transformations, is sent in 10ul TE. A document with the complete plasmid sequence and confirmatory sequencing data of modified areas is emailed along with raw ABI sequencing files.
Discussing your cloning project
Islandia, NY 216-645-1868 email@example.com