Q: What if I don't have source plasmids for the cloning?

A: Specify what gene or destination plasmid you need and indicate that you don't have source plasmids. 

Q: How is my project cost determined?

A: Guidelines for standard cloning costs are provided here. Cloning to viral vectors can increase the cost by $100. Presence or repetitive or high GC sequences, cloning involving large sequences  or multiple changes may also increase the cost.            

Q: How can I contact you to discuss the project’s details?

A: Best way is sending email to service@customdnaconstructs.com you can also call at 216-645-1868.

Sending source material and receiving constructed plasmids   

Q: How and where should I send my source plasmids?

A: Click  here

Frequently asked questions: discussing and executing an order

Q: What data will you need to determine the cost and to execute the project?

A: Full sequence of your destination plasmid and description of the changes you want made (mutagenesis; deletions, tags addition, insert cloning etc.). For insert cloning please send the sequence of the insert, or, better, the complete sequence of the source plasmid for the insert. You can also describe the required changes by simply sending the full sequence of the desired final plasmid. If you don't have complete sequences send the information you have (maps, partial sequences etc.).   

Q: How long will it take to complete the cloning?

A: Standard projects usually take 2-3 weeks but there is no guaranty as unexpected delays some times happen. 

Q: What form of plasmid and documentation will I receive?

A: Plasmids are prepared as miniprep DNA, a small amount, sufficient for multiple transformations, is sent in 10ul TE. A document with the complete plasmid sequence and confirmatory sequencing data of modified areas is emailed along with raw ABI sequencing files.    

Q: What forms of payment you accept? 

A: Purchase orders, checks, direct deposits and credit cards. Domestic credit card payments incur 3% fee and international 4% fee.            

Q: Do I have to pay in advance?  

A: Purchase orders are invoiced only after the project was successfully completed and confirmed by sequencing. If purchase order could not be issued payment is usually collected in advance.

Discussing your cloning project

Q: Will my information kept confidential?

A: Absolutely, the information is used only to facilitate cloning, non of the information will be disclosed to other parties.    

Custom DNA constructs plasmid cloning company

Project cost and payment 

Q: How should I send project information (sequences, maps, instructions) to you?

A: By email to service@customdnaconstructs.com in the text part or as attached files.    

 Islandia, NY      216-645-1868                  service@customdnaconstructs.com